How Ancient Bones Expose The Role Of Humans In Megafauna Collapse

How Ancient Bones Expose The Role Of Humans In Megafauna Collapse


June 20, 2025 | Jane O'Shea

How Ancient Bones Expose The Role Of Humans In Megafauna Collapse


Mystery Of Extinction

Sometimes the most important discoveries come in unexpected ways. Recently, paleontologists solved one of Earth's biggest puzzles using overlooked bones sitting in storage rooms. So, what do they reveal?

Mystery Of Extinction

Prehistoric Paradise

Fifty thousand years ago, North America supported incredible megafauna diversity, rivaling that of modern Africa. Columbian mammoths, weighing up to 10 tons, roamed alongside American mastodons, giant ground sloths exceeding 1,000 kilograms, saber-toothed cats, dire wolves, and house-sized giant beavers in thriving ecosystems.

File:Mammut americanum Sergiodlarosa.jpgSergiodlarosa, Wikimedia Commons

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Human Arrival

This ancient world began changing when the first humans crossed the Bering land bridge around 15,000 BCE. These skilled Paleoindian hunters brought along sophisticated stone tool technology and organized hunting strategies that would dramatically alter the continent's ecological balance forever.

File:Bering Land Bridge NPS 06292023 (53042106205).jpgAlaskaNPS, Wikimedia Commons

Naive Prey

North American mammals proved fatally unprepared for human predators. Unlike African species that evolved alongside hominids, these animals lacked defensive behaviors against bipedal hunters. Mammoths and ground sloths exhibited no fear responses, rendering them exceptionally vulnerable to systematic poaching pressure.

File:Paleontological landscape painting, White Sands National Park, United States.jpguncredited National Park Service (NPS) artist, Wikimedia Commons

Clovis Culture

The transformation accelerated when the distinctive Clovis culture emerged by 13,000 BCE. These individuals were expert hunters who developed specialized fluted spear points perfectly designed for taking down massive prey. They spread their influence from Alaska to Mexico throughout the continent.

File:Son of Clovis.jpgAnthonywpark, Wikimedia Commons

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Bone Isotope Analysis

Bone isotope analysis has provided fascinating insights into the seasonal patterns of human predation on mammoths during the Late Pleistocene. By examining stable isotopes, such as oxygen, carbon, and nitrogen, preserved in mammoth bones and teeth, scientists can reconstruct aspects of the animals' diets and migration patterns.

Bone Isotope AnalysisUnderstanding ancient diets with stable isotope analysis by Brighton & Hove Museums

Seasonal Hunting

So, research showed that many mammoth kills occurred primarily during the winter months. During this season, mammoths were often physically weakened due to terrible environmental conditions and limited food availability. Additionally, they tended to congregate around scarce water sources, making them accessible targets for hunters.

File:Wooly Mammoths.jpgCharles Robert Knight, Wikimedia Commons

Social Organization

Successful megafauna poaching required exceptional levels of human cooperation and planning. Locations such as the Lehner and Murray Springs sites in Arizona and the Colby site in Wyoming exhibit extensive remains of enormously large animals, often with multiple individuals processed simultaneously.

File:Murray Springs Clovis site, ramada 1.JPGAmmodramus, Wikimedia Commons

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Nutritional Impact

Each successful mammoth kill delivered enormous caloric resources for early American populations. A single adult mammoth yielded approximately 4,000 pounds of meat, which was enough protein to sustain dozens of people for months. Fat-rich meat was critical for surviving harsh winters.

File:Woolly mammoth model Royal BC Museum in Victoria.jpgThomas Quine, Wikimedia Commons

Fire Management

Archaeological evidence suggests early humans also changed landscapes through controlled burning. Charcoal layers in sediment cores coincide with the disappearance of megafauna. This indicates that habitat modification through fire may have contributed greatly to extinction pressures beyond direct hunting activities.

Fire Management USEPA Environmental-Protection-Agency, Wikimedia Commons

Population Crashes

It is believed that large herbivores with slow reproduction rates crashed first, followed by carnivores that depended on them for prey. This cascading effect amplified extinction rates throughout interconnected food webs as entire ecosystems collapsed under unprecedented human pressure.

File:Rostock Zoo - 13.JPGThis photograph was taken with a Canon PowerShot S100 by Jed, Wikimedia Commons

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Mass Extinction

What happened next was catastrophic. Between 13,800 and 11,400 BCE, thirty-five genera of large mammals vanished completely within just two millennia. This represented around 72 percent of all megafauna species and was the most severe extinction event since the disappearance of the dinosaurs.

File:Megafauna influence on European vegetation (cropped).jpgHodari Nundu, Wikimedia Commons

Global Pattern

This wasn't an isolated tragedy, as similar extinctions followed human migration worldwide with striking consistency. Australia lost approximately 88% of its megafauna after human colonization, with eradications occurring soon after humans arrived between 65,000 and 44,000 years ago.

File:European Last Interglacial landscapes (cropped).jpgHodari Nundu, Wikimedia Commons

Global Pattern (Cont.)

Furthermore, after human colonization, South America saw one of the worst megafaunal extinctions, losing roughly 83% of its large animal species. The timing of these disappearances closely matches the arrival of modern humans in South America, which occurred around 15,000 years ago.

File:Map of South America, 1593.jpgGerard de Jode, Wikimedia Commons

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Evidence Buried

As these defunct giants began to disappear, they left behind scattered bone fragments across ancient grounds. Over millennia, these remains became fossilized in caves, tar pits, and sedimentary deposits, preserving critical evidence of both animals as well as their interactions with humans.

Evidence Buried Nearly Complete Mammoth Skeleton Found on Michigan Soy Farm | Mashable by Mashable

Evidence Buried (Cont.)

Tar pits, such as the La Brea Tar Pits in Los Angeles, trapped and preserved the bones of mammoths, saber-toothed cats, giant sloths, and other species in remarkable detail. It sometimes even included traces of plants, insects, and wood from their habitats.

File:La Brea Tar Pits January 2021.jpgDowntowngal, Wikimedia Commons

Natural Traps

Caves like Natural Trap Cave in Wyoming and others in North America, Mexico, and Australia have also acted as natural traps or pitfall sites. Similarly, at locations like Naracoorte Caves in Australia, sedimentary layers preserve a continuous fossil record spanning several ice ages.

File:My Public Lands Roadtrip- Natural Trap Cave in Wyoming (19975454095).jpgBureau of Land Management, Wikimedia Commons

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Archaeological Discovery

Beginning in 1934, archaeologists initiated systematic excavations at Late Pleistocene sites across Colorado, most notably at the Lindenmeier site. These early investigations uncovered many bone fragments and artifacts. However, most of the specimens were too weathered and fragmented for precise identification.

File:Soapstone-LindenmeierSite.JPGSethant, Wikimedia Commons

Lamb Spring Discovery

The iconic 1961 excavation at Lamb Spring became legendary in paleontological circles. Photographs show Ed Lewis, Waldo Wedel, and Glenn Scott carefully extracting mammoth bones, wrapping them in protective plaster jackets for preservation. This technique is still used today for fragile specimens.

File:Lamb Spring Site.JPGJeffrey Beall, Wikimedia Commons

Museum Storage

So, these mysterious bone fragments were transferred to storage boxes at the Smithsonian National Museum of Natural History. For decades, they remained unidentified. Their scientific value was seemingly lost due to poor preservation and the limitations of conventional archaeological methods.

File:Smithsonian Institution National Museum of Natural History (7508870948).jpgAlex Proimos from Sydney, Australia, Wikimedia Commons

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Research Team

Ultimately, the interdisciplinary study brought together expertise from multiple institutions in 2024. Dr Mariya Antonosyan from the Max Planck Institute of Geoanthropology, Dr Torben Rick from the Smithsonian, and Prof Nicole Boivin collaborated to bridge molecular archaeology with traditional paleontology.

Research Team Closing Remarks: Dr. Torben Rick by The National Socio-Environmental Synthesis Center

Scientific Breakthrough

As a solution, in recent decades, researchers have developed Zooarchaeology by Mass Spectrometry (ZooMS), a groundbreaking technique that analyzes ancient collagen proteins to identify animal species from highly fragmented or weathered bone remains. ZooMS works by extracting Type I collagen from bone.

Scientific Breakthrough An Introduction to ZooMS by University of York, Dept of Archaeology

Scientific Breakthrough (Cont.)

This is then digested into peptides, and with the help of mass spectrometry, it is used to measure the resulting peptide “fingerprints”. These molecular fingerprints are compared to reference libraries, allowing for taxonomic identification even when traditional morphological features are absent or unrecognizable.

Scientific Breakthrough (Cont.)An Introduction to ZooMS by University of York, Dept of Archaeology

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Modern Analysis

Finally, scientists could unlock the secrets these forgotten specimens held. Despite the poor condition of the legacy bones, which were excavated over a century ago, researchers found that 80% of the fragments retained sufficient preserved collagen for analysis. This high rate enabled successful taxonomic identification.

Untitled Design - 2025-05-29T151241.893Pt1 of 18 on The Mudfossil Discovery and Research Changing History and Evolution Theory by Mudfossil University

Modern Analysis (Cont.)

But that's not all. Of the bone fragments analyzed from six Late Pleistocene archaeological sites, 73% were also successfully classified to the genus level. This identification underlined the presence of key megafauna, specifically mammoth (Mammuthus), bison (Bison), and camelids (camels).

Modern Analysis (Cont.)Pt1 of 18 on The Mudfossil Discovery and Research Changing History and Evolution Theory by Mudfossil University

Butchery Evidence

The ZooMS analysis similarly revealed shocking details hidden within these ancient bones. Several mammoth specimens displayed clear-cut marks, tool scratches, and systematic breaking patterns. All of this proved definitively that Clovis hunters actively butchered these massive creatures rather than simply scavenging from natural deaths.

File:Ranger Holding Mammoth Tooth (37125060224).jpgGreat Sand Dunes National Park and Preserve, Wikimedia Commons

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Butchery Evidence (Cont.)

Note that these findings are supported by high-resolution imaging and microscopic analysis. The same showcased bones fractured by blunt-force trauma, ribs snapped off the vertebrae, puncture marks likely made to extract marrow, and cut-like indications consistent with stone tool use.

Butchery Evidence (Cont.)An Introduction to ZooMS by University of York, Dept of Archaeology

Makeshift Tools

Furthermore, bone flakes and microflakes with sharp edges were also spotted. These are interpreted as makeshift knives used to process mammoth meat, as well as the presence of large rocks among the bones that may have been used as devices for breaking bones.

File:Clovis spearpoints - Cleveland Museum of Natural History.jpgTim Evanson, Wikimedia Commons

Hunting Technology

This evidence also aligned well with archaeological findings across North America. Excavations had already uncovered Clovis spear points embedded in mammoth and mastodon bones. It showed how these sophisticated fluted projectiles provided the technological advantage necessary for bringing down multi-ton animals.

File:Clovis Point.jpgLocutus Borg, Wikimedia Commons

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Hunting Technology (Cont.)

Apparently, the design of Clovis points, characterized by their fluted bases and razor-sharp edges, gave Clovis folks a technological advantage, enabling them to target and process large prey effectively. Some debate remains about how frequently these weapons were used to kill versus butcher megafauna.

File:Stone points of the paleo-Indians, Anthropos, Brno, 187952.jpgZde, Wikimedia Commons

Rapid Collapse

The bone analysis confirmed just how swift this ecological catastrophe had been. Within the last three thousand years, at most, North American ecosystems have gone from megafauna-rich environments to the relatively impoverished fauna we recognize today. It represents an unprecedented rate of species loss.

File:Dry Creek Site (20537070012).jpgNational Park Service, Alaska Region, Wikimedia Commons

Tipping Point

Intense human hunting pressure combined with environmental stress led to an irreversible ecological fall for many megafauna species. As human populations expanded and hunting techniques improved, large animal populations were driven below critical thresholds. They were unable to replenish their numbers quickly.

File:MprimigeniusAalborg1.jpgHonymand, Wikimedia Commons

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Climate Investigation

But could climate change explain such devastation? Scientists examined this possibility thoroughly, discovering that previous ice ages and interglacial periods hadn't triggered similar megafauna losses. According to reports, these same creatures had survived multiple dramatic climate cycles in the past without experiencing mass eradications.

File:Köppen-Geiger Climate Classification Map (1980–2016) no borders.pngBeck, H.E., Zimmermann, N. E., McVicar, T. R., Vergopolan, N., Berg, A., & Wood, E. F., Wikimedia Commons

Selective Targeting

The extinction pattern of the Late Pleistocene provides a critical clue about its underlying causes: it was highly size-selective. Massive mammals, specifically those weighing over 44 kilograms, were systematically eliminated, while smaller mammals, birds, and reptiles generally survived the transition with little impact from environmental changes.

File:Pleistocene SA.jpgDiBgd at English Wikipedia, Wikimedia Commons

Selective Targeting (Cont.)

This extreme size bias distinguishes the Late Pleistocene extinction from earlier events, in which large body size did not render mammals more vulnerable to extinction. The pattern held true within different mammalian orders, indicating that the extinction was not simply due to shared ecological or life-history traits.

File:Museum of Archeology, Ethnography and Ecology of the Siberia - 34.jpgVyacheslav Bukharov, Wikimedia Commons

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Island Refugia

Further proof started to come from isolated populations that survived much longer without human contact. Woolly mammoths persisted on Wrangel Island until 4,000 years ago, while dwarf elephants survived on Mediterranean islands until 4,000–7,000 BCE. This proved that climate wasn't the extinction driver.

File:Woolly mammoths.jpgCharles Robert Knight, Wikimedia Commons

Island Refugia (Cont.)

Scientific studies also show that such isolated populations remained demographically stable for millennia, despite their small size and genetic challenges. The abrupt disappearance of the Wrangel Island mammoths, for instance, occurred well after the end of the last Ice Age and coincided with the arrival of humans.

File:Knight with a Mammoth tusk found on Wrangel Island.jpgVilhjalmur Stefansson, Wikimedia Commons

African Exception

The global extinction pattern of megafauna strongly reinforces the role of human causation. Africa experienced the smallest losses of large mammals during the Late Pleistocene, retaining species such as elephants, rhinoceroses, and large cats. This is widely attributed to the fact that African megafauna evolved alongside early hominids.

File:Two square-lipped rhinoceroses feeding - DPLA - 2a89671734cdaf3f1a96a9c94396f4f4.jpgGarst, Warren, 1922-2016, photographer, Wikimedia Commons

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African Exception (Cont.)

As a result, these species developed natural wariness and defensive behaviors in response to persistent predation pressure from humans and their ancestors. In contrast, on continents where people arrived suddenly and without a long evolutionary history, megafauna were far more vulnerable and suffered catastrophic losses.

File:Woolly rhinoceros (Coelodonta antiquitatis) - Mauricio Antón.jpgMauricio Antón, Wikimedia Commons

Eurasian Climate

In colder and more northerly regions of Eurasia, climate played a stronger role in megafauna extinctions. Environmental changes, such as shifting vegetation, habitat fragmentation due to glaciation, and fluctuating sea levels, created additional stress for megafauna populations alongside human hunting pressure.

File:Parque estatal Chugach, Alaska, Estados Unidos, 2017-08-22, DD 77.jpgDiego Delso, Wikimedia Commons

Ecological Consequences

All of these revelations helped scientists understand the large ecosystem changes that followed. Big herbivores had maintained grasslands through grazing, dispersed seeds over vast distances, and provided essential nutrient cycling. Their disappearance fundamentally altered vegetation and terrain structure permanently.

File:Milwaukee Public Museum November 2022 159 (Africa--Southern Africa--Southern Grasslands- The White-Tailed Gnu).jpgMichael Barera, Wikimedia Commons

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Protein Preservation

The success of this research hinged on the remarkable molecular preservation found within these ancient bone specimens. Despite decades, sometimes over a century, of suboptimal storage conditions in museum collections and field sites, collagen proteins survived in 80 percent of the tested bones.

File:02021 0014 Woolly mammoth bones found in Przemysl Region.jpgSilar, Wikimedia Commons

Reference Gaps

A significant limitation, though, emerged in species identification capabilities. ZooMS reference libraries remain well-developed for Eurasian fauna but severely lacking for North American species. This causes great challenges when researchers attempt to match mass spectrometry results with known protein signatures.

File:Elizabeth Reitz in Zooarchaeology Laboratory in Georgia Museum of Natural History.jpgBpavaoz, Wikimedia Commons

Museum Revolution

On a good note, the breakthrough had a positive impact on scientific perspectives on museum collections worldwide. Thousands of seemingly worthless bone fragments in storage facilities now represent potential goldmines of invaluable data, which is about extinct species, past ecosystems, and the precise timing of prehistoric extinctions.

File:Woolly mammoths by Knight.jpgCharles R. Knight, Wikimedia Commons

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Conservation Implications

Moreover, the research findings carried urgent warnings for modern conservation efforts. Today's remaining megafauna face similar pressures from human activities, habitat loss, and climate change. Historical extinction trends offer valuable insights for protecting elephants, rhinoceroses, and other endangered creatures.

File:Elephant running.jpgByrdyak, Wikimedia Commons


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